Non-radioactive LATS in vitro Kinase Assay

A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)

While many methods exist to quantitatively determine protein kinase activities, 32P-based radioactive assays remain the workhorse of many laboratories due to their high sensitivity, high signal to noise ratio, lack of interference by fluorescent and light-absorbing small molecules, and easy quantitation. Here, we demonstrate that the interaction between the yeast Rad53 Forkhead-associated (FHA)...

NON-RADIOACTIVE ASSAY FOR ACETYL-CoA CARBOXYLASE ACTIVITY

An ELISA DYRK1A non-radioactive assay suitable for the

The DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1A) gene encodes a proline-directed Ser/Thr kinase. Elevated expression and/or altered distribution of the kinase have been implicated in the neurological impairments associated with Down syndrome (DS) and Alzheimer’s disease (AD). Consequently, DYRK1A inhibition has been of significant interest as a potential strategy for t...

Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer.

Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequencing, it has not been used in the in vitro primer extension assay. ...

Radioactive Waste Assay for Reclassification

Following the ban on deep sea disposal of radioactive wastes in 1983 the Atomic Weapons Establishment (AWE) in the United Kingdom (UK) has stored plutonium (Pu) and uranium (U) contaminated wastes on site in 200 l steel drums or as wrapped packages containing filters or other materials. This was because of difficulty in demonstrating compliance with the with the 100 Bq/g Pu alpha activity limit...